Practice Parameter’s and Middleton’s Allergy: Principles and Practice both recommend 10 mg/ml as the “preferred positive control for prick-puncture tests.” 2,3 This is the exact concentration in our Histamine Positive Control.

Skin Prick Testing and the Use of Histamine References recommends “histamine 10 mg/mL as an international positive reference.”4

“Histamine Dihydrochloride at 10mg/mL (equivalent to 6 mg/mL Histamine base) has been shown to elicit fewer false negative and false positive reactions”5 using the same skin testing device than 1mg/mL histamine base.

7099ED Histamine for Percutaneous (Scratch, Prick or Puncture) Administration. Histamine Dihydrochloride 10 mg/mL (equivalent to 6 mg/ml Histamine base) 5 mL


1 Nordic Council on Medicines: Registration of Allergen Preparations. Nordic Guidelines, ed 1. Uppsala, NLN Publications, 1982, No 7, pp 16.

2 Berstein, I.L. et al. Practice parameters for the diagnosis and treatment of asthma. Ann Allergy Asthma lmmunol. 2008; 96:S1- S148.

3 N.F. Adkinson, B. B. (2014). Middleton’s Allergy: Principles and Practice. 8th ed., 1123.

4 H.J. Malling. Skin Prick Testing and the Use of Histamine References. Allergy, 39: 596-601, (1984).

5 R.B. Berkowitz, D.G. Tinkleman, D. Lurz, et al. Evaluation of the Multi-test Device for Immediate Hypersensitivity Skin Testing. J. Allergy Clin. Immunol. 90: 979-985 (1992)